Journal: Pharmaceuticals

Article Title: Xiebai San Alleviates Allergic Pulmonary Inflammation by Modulating Arachidonic Acid Metabolism

doi: 10.3390/ph19030440

Figure Lengend Snippet: Effects of XBS on the p38 MAPK pathway in mice. ( A ) Representative Western blot images and quantitative analysis of p-p38 MAPK/p38 MAPK, COX-2, and 15-LOX protein expression in lung tissues collected 24 h after the final OVA challenge (day19). ( B ) Representative Western blot images and quantitative analysis of p-p38 MAPK/p38 MAPK, COX-2, and 15-LOX protein expression in lung tissues collected 30 min after C48/80 injection. Protein expression levels were normalized to GAPDH and presented as mean ± SD ( n = 6 per group). Dashed lines indicate proteins detected on separate membranes due to the similar molecular weights of COX-2 (74 kDa) and 15-LOX (75 kDa). * p < 0.05 and ** p < 0.01 compared with the control group; # p < 0.05 and ## p < 0.01 compared with the model group.

Article Snippet: Antibodies against p38MAPK (8690), Phospho-p38MAPK (Thr180/Tyr182) (4511), COX-2 (12282), and rabbit polyclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH), were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Expressing, Injection, Control